Homogeneous cell lines are essential in industry and research if reliable and reproducible data are to be obtained. The Spodoptera frugiperda (Sf-9) cell line routinely used for the production of recombinant proteins was found to be heterogeneous, containing a mixture of diploid and tetraploid cells.
WhatsAppHematopoietic stem cells display heterogeneous, ... These include cells that have distinctive behaviors in terms of cell production and lineage bias (Dykstra et al., 2007; ... A HUe founder line with 20 copies of transgene inserted can have 10 3 color combinations. (C) Testing the efficiency of expression of fluorescent proteins by crossing …
WhatsAppEvolution from adherent to suspension: systems biology of ...
WhatsAppCell heterogeneity (due to genetic variation, mixture of different cell types including normal cells, transdifferentiation, etc.) is responsible for cancer heterogeneity. Therefore the optimal determination of cancer subtypes necessitates the detection of the many forms of cell heterogeneity on a per-cell basis, which requires careful evaluation of appropriate …
WhatsAppHuman cancer cell lines have long served as tools for cancer research and drug discovery, but the presence and the source of intra-cell-line heterogeneity remain elusive. Here, we perform single ...
WhatsAppA novel heterogeneous network-based method for drug ...
WhatsAppMacrophages are critical regulators of immunity and play key roles in a range of biological and pathological processes. This review provides a conceptual framework for improving our understanding of the contributions of macrophages of different origins to physiological and disease states.
WhatsAppAn assembly cell line (ACL) is one type of cell production practice, derived from the Toyota Production System in the electronics industry and rapidly spread to other fields.
WhatsAppThis research is motivated by a mechatronic device production company J, which applied a type of cell production named assembly cell line (ACL) in the final assembly. In this mode, the …
WhatsAppThe Sf9 cell line is broadly used for manufacturing baculovirus-expressed viral vaccines. We previously reported the presence of a novel, rhabdovirus in the Sf9 cell line, which contained a unique X gene (Sf-rhabdovirus; designated as X + in this paper). These results were extended by other reports describing an Sf-rhabdovirus variant in Sf9 cells, which …
WhatsAppWound healing, fibroblast heterogeneity, and fibrosis
WhatsAppSingle cell multi-omics reveal intra-cell-line heterogeneity ...
WhatsAppThe time taken for the development of biopharmaceutical protein production cell lines can be reduced via flow cytometry, where it is utilized as a high-speed cell …
WhatsAppThe bulk output of an immune response represents the combined behaviors of a highly diverse ensemble. Many unique subsets of cells work together to fight a range of potential threats, maintain long-term memory, and establish tolerance [1,2]. Moreover, the interplay between these groups of cells establishes checks and balances, …
WhatsAppMore than 20 years after tPA approval, CHO cells remained as the preferred mammalian cell line for the production of …
WhatsAppQuantified process parameters in pharmaceutical production and in mammalian cell fermentation process development only represent culture average values and do not portray the heterogeneity …
WhatsAppSuch culture comprises mostly heterogeneous cells, and most cells divide only for a limited time. However, these cells are much similar to their parents. Depending on their origin, primary cells grow either as an adherent monolayer or in a suspension. ... Applications of Cell Line. A. Vaccines Production.
WhatsApp1.. IntroductionIn the just-in-time (JIT) production system, a single-unit production and conveyance system is applied to a production line without conveyors which manufactures different kinds of relatively small parts. To achieve this at a low production cost, a U-shaped layout is used with multi-function workers. When the same …
WhatsAppA stem cell roadmap of ribosome heterogeneity reveals ...
WhatsAppIn this chapter, we focus on the development of cell lines for production of biopharmaceuticals, exemplified by CHO cells, which is the most widely used cell type …
WhatsAppSingle-cell RNA sequencing highlights the influence of host–pathogen interactions and stochasticity on transcriptional and phenotypic variance in lymphoblastoid cell lines derived from Epstein–Barr virus-infected primary B cells.
WhatsAppIn the present study, a novel procedure for establishing homogeneous tetraploid embryogenic cell lines in Magnolia officinalis has been established. Embryogenic cell aggregate (ECA) about 100-200 μm across, and consisting of dozens of cells, regenerated into a single colony of new ECAs and somatic embryos following colchicine …
WhatsAppFirst systemic lysosomal proteomes in association with longevity and tissue specificity in C. elegans uncover lysosomal heterogeneity across multiple scales and provide valuable resources for understanding how lysosomes modulate signal transduction, organelle communication, tissue physiology, and organism longevity.
WhatsAppWe use the maximum entropy principle to model the phenotypic distribution of an heterogeneous population of cells in a chemostat. Given the metabolic network …
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